某养鸡场禽霍乱分离菌对常用抗菌药物的敏感性研究

从六安地区某养鸡场禽霍乱病鸡上分离到株禽多杀性巴氏杆菌，采用纸片扩散法和试管稀释法分别测定了青霉素钾、土霉素、硫酸卡那霉素、硫酸庆大霉素、硫酸链霉素、复方新诺明、甲砜霉素、氟哌酸、环丙沙星和恩诺沙星等10种药物对分离菌的体外抗菌活性。结果表明分离到的禽多杀性巴氏杆菌对青霉素钾和土霉素耐药；对硫酸卡那霉素、硫酸庆大霉素、硫酸链霉素、甲砜霉素、氟哌酸敏感；对复方新诺明、环丙沙星和恩诺沙星极敏感。两种测定方法得到的结果一致。因此复方新诺敏、环丙沙星和恩诺沙星可作为目前该发病场防治禽霍乱的首选药物。     

新疆石河子农耕区鼠类群落结构及其危害状况

2001年6～9月,在石河子农耕区依不同生境类型选择9个样地,以夹夜法布夹11 300夹次,共捕获鼠417只,鼠种由小家鼠、灰仓鼠、红尾沙鼠、褐家鼠等10种组成.采用聚类分析方法将鼠类群落划分为3种类型:农田-林地鼠类群落(小家鼠 灰仓鼠 根田鼠)、荒漠-沙地鼠类群落(子午沙鼠 红尾沙鼠 大沙鼠)和城市居民点鼠类群落(褐家鼠 小家鼠 灰仓鼠).其中,农田-林地鼠类群落的Shannon-Wiener多样性指数较高.根据鼠类群落结构特征,并结合鼠害发生状况将垦区鼠类危害型划分为4种类型.     

Influence of APP17 peptide on the learning,memory in SAMP10 mi ce and oxidative stress in mouse mitochondria in hippocampal neurons

AIM:To observe the influence of APP17-mer peptide on the learning and memory,and oxidative stress in mitochondria in hipp ocampal neurons of senescence accelerated mouse/prone 10 (SAMP10).METHODS:The 4-month-old SAMP 10s were randomly divided into mod el group and APP17-mer peptide group (the model group+APP17-mer peptide).The se nescence accelerated mouse/resistance1 (SAMR1) were used for normal control.The APP17-mer peptide group was injected with APP17-mer peptide subcutaneously in a dose of 0.34 μg/per mouse,three times a week;while the model group and the control group were injected with saline.After 28 weeks,water maze test was use d to evaluate the learning and memory of these mice.Then hydroxy lamine reactio n and thiobarbituric acid reaction were used to assay the activity of superoxid e dismutase (SOD) and contents of malondialdehyde (MDA) in mitochondria in hippo campal neurons,respectively.RESULTS:(1) The water maze test showed that the full-course swi mming time and numbers of error committed in model group were significantly incr eased as compared with the normal control (P<0.05).The results in APP17-me r peptide treated group were the same as that in normal control group.(2) Compa red with the normal control,the activity of SOD decreased in model group (P <0.01),and the contents of MDA increased (P<0.01).APP17-mer partially re versed the increase in MDA and the decrease in SOD.The content of MDA was lower and the activity of SOD was higher in APP17-mer peptide group than those in mod el group (P<0.05).CONCLUSION:The learning and memory deficit in SAMP10 are observ ed,and the balance of free radical generation/free radical scavenger is impai red.APP17-mer peptide has an antioxidant character and corrects the abnormal ch ange of SOD and MDA,which may be related to its protective effect of learning and memory.     

食用菌双翅目害虫的采集和玻片标本制作方法

主要提供了食用菌双翅目标本的采集方法和玻片标本的制作方法。标本的采集主要是在菇房用吸虫管采集,或者用毛笔蘸取酒精直接采集;玻片的制作分为5个步骤,即浸解脱色、洗涤、脱水、整姿封盖和干燥。     

多效唑对八仙花组培苗营养生长及成花的影响

以八仙花1、2 a生组织培养苗为试验对象,研究了多效唑不同浓度、不同施药时间、不同施药次数对八仙花组培苗促成栽培中其植株营养生长及成花的影响.结果表明:施用浓度和时间是多效唑抑制八仙花组培苗植株营养生长促进其生殖生长的关键,7月中旬以浓度100～150 mg/L的多效唑溶液对八仙花1、2 a生组织培养苗进行叶面喷施,最有利于其花芽分化,成花率高,且从株型、花形花色等各项指标综合考虑,多效唑对促进八仙花2a生组培苗成花效果更显著.施药次数也影响八仙花组培苗营养生长和成花,连续施药3次对抑制其营养生长促进成花效果最佳.     

成年公兔睾丸支持细胞的分离纯化及鉴定

为了寻求一种从成年公兔睾丸获得大量高纯度、高活率支持细胞的方法，本试验采用了胶原酶、胰蛋白酶和透明质酸酶联合消化法，从成年公兔生精小管分离出支持细胞，通过Tris-HCl低渗处理和选择性贴壁培养对所得的支持细胞进行纯化，并采用形态学和HE染色鉴定。最终获得了大量较高纯度（78.3%）和高活率(90.7%)的支持细胞。     

Effects of cinnamyl aldehyde on c-Fos and c-Myc expression in NIH3T3 cells

AIM: Cinnamyl aldehyde (CA) is one alcohol ingredient derived from Cinnamomum cassia,which is widely used in treating chronic skin wound in Chinese medicine with the curative effect of ‘rescuing YANG’.The purpose of the present study was to investigate the expression of c-Fos,c-Myc proteins at different time points in NIH3T3 treated with CA and explore the possible mechanism of promoting cell proliferation by CA.METHODS: MTT assay was used for observing cell proliferation.Expression of c-Fos and c-Myc proteins in NIH3T3 cells were assessed by immunocytochemistry assay.RESULTS: The cell proliferation was promoted obviously when CA concentration was between 8.8×10^-2 μg/L and 8.8×10 μg/L.CA at concentration of 5.5 μg/L significantly induced expression of c-Fos,c-Myc proteins at 2-3 h after the stimulation compared with control group (P<0.01).CONCLUSION: CA increases expression of c-Fos and c-Myc proteins,which may be one of mechanisms for CA to promote NIH3T3 cell proliferation.     

生长调节剂对黄绿蜜环菌菌丝生长的影响

选用三十烷醇(triacontanol)和肌醇(inositol)两种生长调节剂对黄绿蜜环菌(Armillaria luteo-virens)菌丝生长的影响进行研究。结果表明:三十烷醇和肌醇对菌丝生长都有较明显的影响,但肌醇效果不如三十烷醇。在培养基中添加1.5ⅹ10-7mg.mL-1三十烷醇,可明显提高菌丝生长速度,缩短培养周期。     

脱硫石膏改良碱土过程中特征值变化的研究

本文通过田间试验,研究了脱硫石膏在改良碱土过程中对碱土特征值(盐分组成、pH值、碱化度、土壤渗透性及硬度)的影响。结果表明:施用脱硫石膏后,碱土各特征值都发生了显著变化,主要表现在碱化度从改良前的30%以上降低到了改良后的5~10%之间;pH值从改良前的9.4以上降低到了改良后的8.2以下;碳酸根、重碳酸根离子明显减少,硫酸根、钙离子及钠离子含量有所增加;土壤渗透性提高了3~8倍;土壤硬度测定值虽仍偏大,但却表现为随着脱硫石膏的施入而减小的趋势。     

广东省大学生运动休闲消费特征研究

为提高潜水蒸发计算精度，利用潜水蒸发强度、埋深为零时的土面蒸发强度和水面蒸发强度的实测资料对阿维里扬诺夫公式、叶水庭公式和雷志栋公式中E₀值的选取进行了探讨，同时对比分析了E₀分别为水面蒸发强度和埋深为零时土面蒸发强度两种情况下的潜水蒸发强度。结果表明，针对新疆地区粘壤土类型，对于上述三种公式，若用埋深为零的土面蒸发强度代替水面蒸发强度，计算结果会产生误差，精度偏低，应选用E₀为水面蒸发强度进行计算，获得了η与地下水埋深的指数函数关系。该研究为潜水蒸发经验模型中E₀值的选取提供参考，同时为求解雷志栋公式中参数η提供一种新方法。